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L'intégrase IntI1, dont l'expression est régulée par la réponse SOS bactérienne, catalyse l'insertion/excision des cassettes de gène. Les intégrons de classe 1 sont des systèmes bactériens capables de capturer, échanger et exprimer des cassettes de gène de résistance aux antibiotiques. Ces derniers sont très tolérants aux antibiotiques et à l’origine d’infections persistantes difficiles à traiter. We foresee that MuViScopy will open the path to better understand developmental processes at the whole organism scale in living systems that necessitates gas exchange by ventilation.ĭans les écosystèmes naturels les bactéries vivent sous forme de biofilm. We illustrate the capacity of MuViScopy by live-imaging Drosophila melanogaster pupal development throughout metamorphosis, highlighting how internal organs are formed and multiple organ development is coordinated. MuViScopy enables live-imaging of multiple organs with cellular resolution using sample rotation and confocal imaging without the need of sample embedding. Here, we implemented a multi-view multi-scale microscopy strategy based on confocal spinning disk microscopy, called Multi-View confocal microScopy (MuViScopy). Yet, these advances mainly rely on mounting the specimen in agarose or aqueous solutions, precluding imaging of organisms whose oxygen uptake depends on ventilation. Over the last decade numerous advances have been made in live-imaging, enabling high resolution imaging of whole organisms at cellular resolution. Understanding how development is coordinated in multiple tissues and gives rise to fully functional organs or whole organisms necessitates microscopy tools.
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